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ASCP ASCP-MLT

ASCP-MLT

Exam Code: ASCP-MLT

Exam Name: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)

Updated: Jul 30, 2026

Q&A Number: 562 Q&As

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ASCP ASCP-MLT Exam Syllabus Topics:

SectionObjectives
Topic 1: Blood Banking / Immunohematology- Blood group systems and antibody identification
  • 1. Crossmatching procedures
    • 2. ABO and Rh systems
      Topic 2: Hematology- Blood cell analysis and disorders
      • 1. Anemias and leukemias overview
        • 2. CBC and differential interpretation
          Topic 3: Laboratory Operations- Quality and safety in the clinical lab
          • 1. Laboratory safety and regulations
            • 2. Quality control and assurance
              Topic 4: Microbiology- Clinical microbiological techniques
              • 1. Culture and sensitivity testing
                • 2. Bacteriology and organism identification
                  Topic 5: Urinalysis and Body Fluids- Fluid analysis and interpretation
                  • 1. CSF and other body fluids
                    • 2. Urine chemical and microscopic examination
                      Topic 6: Clinical Chemistry- Metabolic and analytical testing
                      • 1. Endocrine and metabolic panels
                        • 2. Enzymes and electrolytes

                          ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

                          1. Free T4 is affected the least by changes in plasma proteins during pregnancy.
                          Which of the following thyroid function assays is affected least by pregnancy:

                          A) Total T4
                          B) Total T3
                          C) Free T4


                          2. This antibody is found in the serum of Le(a-b-) secretors.
                          Which one of these Lewis blood group system phenotypes usually produces anti-Lea?

                          A) Le(a--b+)
                          B) Le(a+b-)
                          C) Le(a--b-)
                          D) Le(a+b+)


                          3. Hemoglobin electrophoresis uses an electric field to separate hemoglobin molecules based on differences in net electrical charge. The rate of electrophoretic migration is also dependent on the ionic radius of the molecule, the viscosity of the solution through which it is migrating, the electrical field strength, temperature, and the type of supporting medium used.
                          Electrophoretic separation of hemoglobin fundamentally relies on:

                          A) Net charge differences of molecules
                          B) Concentration differences of molecules
                          C) Size differences of molecules
                          D) Shape variations of molecules


                          4. Kell, Kidd, and Duffy are usually warm reacting IgG antibodies. The most common P system antibody is anti-P1, which is a frequently naturally occurring low titer IgM antibody, seen in most P2 individuals.
                          Blood bank
                          Which of the following blood groups is most frequently associated with cold agglutinins:

                          A) Duffy
                          B) Kidd
                          C) P
                          D) Kell


                          5. A dilution commonly used for a routine sperm count is a 1:20.
                          A dilution commonly used for a routine sperm count is:

                          A) 1:20
                          B) 1:400
                          C) 1:200
                          D) 1:2


                          Solutions:

                          Question # 1
                          Answer: C
                          Question # 2
                          Answer: C
                          Question # 3
                          Answer: A
                          Question # 4
                          Answer: C
                          Question # 5
                          Answer: A

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